Nuclear factor-kappaB regulates the transcription of NADPH oxidase 1 in human alveolar epithelial cells

Abstract Objective Acute lung injury (ALI) is characterized by inflammation and oxidative stress. Nuclear factor-kappaB (NF-κB) mediates the expression of various inflammation-related genes, including NADPH oxidase family. This study aimed to identify potential regulatory role NF-κB on oxidases in tumor necrosis factor-α (TNF-α)-induced stress human alveolar epithelial cells. Methods A549 cells were treated with TNF-α for 24 h establish ALI cell models. RT-PCR, western blot, assessment stress, Alibaba 2.1 online analysis, electrophoretic mobility shift assays luciferase reporter analysis employed TNF-α-induced Results The NF-κB/p65 was notably upregulated TNF-α-stimulated knockdown siRNA significantly inhibited Moreover, could inhibit activation NOX1, NOX2 NOX4 mRNA protein next demonstrated that activated transcription NOX1 binding -261 -252 bp (NOX1/κB2, TAAAAATCCC) region promoter Conclusion Our data can aggravate regulating response NOX4. promote transcriptional activity via its